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Genome-wide association study of pre-harvest sprouting resistance in Chinese wheat founder parents Genet. Mol. Biol.
Lin,Yu; Liu,Shihang; Liu,Yaxi; Liu,Yujiao; Chen,Guoyue; Xu,Jie; Deng,Mei; Jiang,Qiantao; Wei,Yuming; Lu,Yanli; Zheng,Youliang.
Abstract Pre-harvest sprouting (PHS) is a major abiotic factor affecting grain weight and quality, and is caused by an early break in seed dormancy. Association mapping (AM) is used to detect correlations between phenotypes and genotypes based on linkage disequilibrium (LD) in wheat breeding programs. We evaluated seed dormancy in 80 Chinese wheat founder parents in five environments and performed a genome-wide association study using 6,057 markers, including 93 simple sequence repeat (SSR), 1,472 diversity array technology (DArT), and 4,492 single nucleotide polymorphism (SNP) markers. The general linear model (GLM) and the mixed linear model (MLM) were used in this study, and two significant markers (tPt-7980 and wPt-6457) were identified. Both markers...
Tipo: Info:eu-repo/semantics/article Palavras-chave: General linear model; Linkage disequilibrium; Marker-trait associations; Mixed linear model; Seed dormancy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400620
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Up-regulated miR-106b inhibits ox-LDL-induced endothelial cell apoptosis in atherosclerosis BJMBR
Zhang,Yunqing; Wang,Li; Xu,Jie; Kong,Xiaomei; Zou,Lin.
This research aimed to explore the molecular mechanism of microRNA (miR)-106b in cell apoptosis of atherosclerosis (AS). Human aortic endothelial cells (HAECs) were divided into control group, oxidized-low-density lipoproteins (ox-LDL) group, miR-106b NC+ox-LDL group, miR-106b mimics+ox-LDL group, miR-106b mimics+PTEN+ox-LDL group, and miR-106b mimics+empty+ox-LDL group. Real-time fluorescence quantitative polymerase chain reaction, cholecystokinin, TdT-mediated biotinylated nick end-labeling assay, luciferase reporter gene assay, and flow cytometry analysis were performed to determine the morphology, proliferation, and apoptosis in HSECs. Moreover, the levels of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), Bcl-2, p-P13K, and p-AKT in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atherosclerosis; Ox-LDL; MiR-106b; PTEN; P13K/AKT; Endothelial cell apoptosis.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300610
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